Further studies on partially purified calf thymus DNA polymerase a

Nucleic Acids Res. 1979 Jul 25;6(10):3341-52. doi: 10.1093/nar/6.10.3341.


Attempts to prevent the urea conversion of a 200-230,000 molecular weight DNA polymerase alpha to a 150-170,000 molecular weight form by the inclusion of protease inhibitors have not been successful. No other method has been found capable of dissociating a 50-70,000 fragment or subunit from the DNA polymerase subunit. Addition of this 50-70,000 subunit to the polymerase subunit does not aid the binding of the enzyme to DNA, but does have an effect on the utilisation of synthetic template-initiator complexes by the polymerase subunit.

MeSH terms

  • Animals
  • Cattle
  • DNA Polymerase II / isolation & purification
  • DNA Polymerase II / metabolism*
  • DNA-Directed DNA Polymerase / metabolism*
  • Kinetics
  • Molecular Weight
  • Structure-Activity Relationship
  • Templates, Genetic
  • Thymus Gland / enzymology*


  • DNA Polymerase II
  • DNA-Directed DNA Polymerase