Purification on renografin density gradients of Chlamydia trachomatis grown in the yolk sac of eggs

Appl Microbiol. 1974 Jan;27(1):102-6. doi: 10.1128/am.27.1.102-106.1974.


Chlamydia trachomatis grown in the yolk sac of embryonated eggs was purified by centrifugation on continuous isopycnic Renografin density gradients. A band of chlamydial particles with a buoyant density of 1.20 contained 70% of the starting particles, and electron microscopy revealed the virtual absence of contaminating egg material. Centrifugation on Renografin gradients caused only a moderate decrease in infectivity. For large-scale purification, infected yolk sac was centrifuged through Renografin solutions, resulting in greater than 60% recovery of starting chlamydial particles, but less than 1% recovery of the dry weight and protein.

MeSH terms

  • Animals
  • Cell Count
  • Centrifugation, Density Gradient*
  • Chick Embryo
  • Chlamydia / growth & development
  • Chlamydia / isolation & purification*
  • Chlamydia / pathogenicity
  • Diatrizoate
  • Female
  • Methods
  • Microscopy, Electron
  • Vitelline Membrane / microbiology


  • Diatrizoate