Articular cartilage preservation and storage. II. Mechanical indentation testing of viable, stored articular cartilage

Arthritis Rheum. 1979 Oct;22(10):1102-8. doi: 10.1002/art.1780221009.

Abstract

Mature rabbit articular cartilage in the form of distal femoral condyles, composite osteoarticular structures, were incubated in the presence of alpha-tocopherol (200 micrograms/ml) over a period of time. Indentation testing and 35S uptake indicate preservation of sustained load carrying capacity and viability, respectively, in the presence of alpha-tocopherol for up to 30 days in organ culture. Condylar cartilage stored in the absence of alpha-tocopherol as well as frozen cartilage demonstrated progressive inability to resist sustained loading over time. Nonoptimal synthetic function apparently occurred in these latter two groups when compared to alpha-tocopherol stored material.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cartilage, Articular / drug effects
  • Cartilage, Articular / physiopathology
  • Cartilage, Articular / transplantation*
  • Culture Media
  • Culture Techniques / methods*
  • Dimethyl Sulfoxide
  • Dose-Response Relationship, Drug
  • Freezing
  • Hexosamines / metabolism
  • Hydroxyproline / metabolism
  • Male
  • Rabbits
  • Stress, Mechanical
  • Time Factors
  • Tissue Preservation / methods*
  • Tissue Survival
  • Vitamin E / pharmacology

Substances

  • Culture Media
  • Hexosamines
  • Vitamin E
  • Hydroxyproline
  • Dimethyl Sulfoxide