Differentiated rat glial cell strain in tissue culture

Science. 1968 Jul 26;161(3839):370-1. doi: 10.1126/science.161.3839.370.


Rat glial tumors, induced by injections of N-nitrosomethylurea, were plated and propagated in culture. Among a few cell strains obtained, one clone contains S-100 protein, which is unique to brain in vertebrates. Stationary-phase cultures contain approximately ten times more S-100 protein per cell than exponentially growing cells. When injected into newborn rats, cells producing S-100 grew as a glial tumor, which contained S-100 protein.

MeSH terms

  • Animals
  • Brain Neoplasms / chemically induced
  • Brain Neoplasms / metabolism*
  • Cell Differentiation*
  • Clone Cells*
  • Culture Techniques*
  • Glioma / chemically induced
  • Glioma / metabolism*
  • Microscopy, Phase-Contrast
  • Neoplasms, Experimental / chemically induced
  • Nerve Tissue Proteins / metabolism*
  • Nitroso Compounds
  • Rats
  • Urea


  • Nerve Tissue Proteins
  • Nitroso Compounds
  • Urea