Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II

Proc Natl Acad Sci U S A. 1971 Apr;68(4):761-4. doi: 10.1073/pnas.68.4.761.


The major DNA-synthesizing enzyme present in Pol A(1) (-)Escherichia coli (DNA polymerase II) has been purified to homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme requires all four deoxynucleoside triphosphates, Mg(++), NH(4) (+), and native DNA for maximal activity. The enzyme activity is sensitive to sulfhydryl reagents and is insensitive to anti-DNA polymerase I antiserum. A second DNA-synthesizing enzyme, present in low amounts, has been identified in Pol A(1) (-) extracts. The relationship of this enzyme to DNA polymerases I and II is discussed.

MeSH terms

  • Cell-Free System
  • Chemical Phenomena
  • Chemistry
  • Chromatography
  • Chromatography, DEAE-Cellulose
  • DNA Nucleotidyltransferases / isolation & purification*
  • DNA Replication*
  • Electrophoresis
  • Escherichia coli / enzymology
  • Histocytochemistry*
  • In Vitro Techniques
  • Mutation
  • Templates, Genetic


  • DNA Nucleotidyltransferases