Inactivation of ribosomes in vitro by colicin E 3

Proc Natl Acad Sci U S A. 1971 Oct;68(10):2421-5. doi: 10.1073/pnas.68.10.2421.

Abstract

Cell-free protein synthesizing extracts incubated with purified preparations of colicin E(3) showed a marked decrease of their protein synthesizing activity. Incubation of ribosomes prepared from S-30 extracts with E(3) resulted in their inactivation. Their 16S RNA was found to lose a terminal fragment, similar in size to the fragment released upon in vivo treatment with E(3). Extracts derived from colicinogenic strains are resistant to the action of E(3)in vitro.

MeSH terms

  • Autoradiography
  • Bacterial Proteins / biosynthesis*
  • Carbon Isotopes
  • Cell-Free System
  • Colicins / pharmacology*
  • Escherichia coli / cytology
  • Escherichia coli / immunology
  • Escherichia coli / metabolism
  • Lysine / metabolism
  • Mutation
  • Phenylalanine / metabolism
  • Polynucleotides / metabolism
  • RNA, Ribosomal / analysis
  • RNA, Ribosomal / biosynthesis*
  • Ribosomes / drug effects
  • Ribosomes / metabolism*
  • Temperature
  • Tritium
  • Uracil / metabolism

Substances

  • Bacterial Proteins
  • Carbon Isotopes
  • Colicins
  • Polynucleotides
  • RNA, Ribosomal
  • Tritium
  • Phenylalanine
  • Uracil
  • Lysine