Abstract
Cell-free protein synthesizing extracts incubated with purified preparations of colicin E(3) showed a marked decrease of their protein synthesizing activity. Incubation of ribosomes prepared from S-30 extracts with E(3) resulted in their inactivation. Their 16S RNA was found to lose a terminal fragment, similar in size to the fragment released upon in vivo treatment with E(3). Extracts derived from colicinogenic strains are resistant to the action of E(3)in vitro.
MeSH terms
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Autoradiography
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Bacterial Proteins / biosynthesis*
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Carbon Isotopes
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Cell-Free System
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Colicins / pharmacology*
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Escherichia coli / cytology
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Escherichia coli / immunology
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Escherichia coli / metabolism
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Lysine / metabolism
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Mutation
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Phenylalanine / metabolism
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Polynucleotides / metabolism
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RNA, Ribosomal / analysis
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RNA, Ribosomal / biosynthesis*
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Ribosomes / drug effects
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Ribosomes / metabolism*
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Temperature
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Tritium
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Uracil / metabolism
Substances
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Bacterial Proteins
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Carbon Isotopes
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Colicins
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Polynucleotides
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RNA, Ribosomal
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Tritium
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Phenylalanine
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Uracil
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Lysine