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. 1969 Jun 1;129(6):1349-65.
doi: 10.1084/jem.129.6.1349.

Human Immunity to the Meningococcus. 3. Preparation and Immunochemical Properties of the Group A, Group B, and Group C Meningococcal Polysaccharides

Free PMC article

Human Immunity to the Meningococcus. 3. Preparation and Immunochemical Properties of the Group A, Group B, and Group C Meningococcal Polysaccharides

E C Gotschlich et al. J Exp Med. .
Free PMC article

Abstract

The group-specific polysaccharides of group A and group C meningococci have been isolated by a new procedure which employs the cationic detergent Cetavlon to precipitate these polysaccharides from the whole culture. The A and C polysaccharide prepared by this method are noteworthy because they are of high molecular weight. The main constituent of the A polysaccharide is N-acetyl, O-acetyl mannosamine phosphate; of the C polysaccharide N-acetyl, O-acetyl neuraminic acid. This purification procedure, when applied to cultures of group B organisms, yields a polysaccharide consisting primarily of N-acetyl neuraminic acid. A passive hemagglutination test developed to measure antibodies to the polysaccharides demonstrated the specificity of these antigens. Using a hemagglutination inhibition test, these antigens were again found to be group-specific, and this test could be used for serogrouping meningococcal isolates.

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