Characterization of the deoxyribonucleic acid of various strains of halophilic bacteria

J Bacteriol. 1969 Jul;99(1):248-54. doi: 10.1128/jb.99.1.248-254.1969.

Abstract

Bacteria classified as extreme halophiles, in the genera Halobacterium and Halococcus, contain deoxyribonucleic acid (DNA) which displays two components in a CsCl equilibrium density gradient. The base composition of the major DNA component ranges from 66 to 68% guanine plus cytosine (GC), whereas that of the satellite DNA comprising some 11 to 36% of the total, is between 57 and 60% GC. Purification of the bacterial cells in a CsCl density gradient and other more conventional strain purification procedures both indicated that the presence of the satellite DNA component is not a result of mixed cultures.

MeSH terms

  • Acridines / pharmacology
  • Bacteria / analysis*
  • Bacteria / drug effects
  • Bacteria / metabolism
  • Centrifugation, Density Gradient
  • DNA, Bacterial / analysis*
  • Escherichia coli / analysis
  • Halobacterium / analysis*
  • Mitomycins / pharmacology
  • Pseudomonas aeruginosa / analysis
  • Rhodopseudomonas / analysis
  • Sodium Chloride / metabolism

Substances

  • Acridines
  • DNA, Bacterial
  • Mitomycins
  • Sodium Chloride