Size changes in single muscle fibers during fixation and embedding

Tissue Cell. 1975;7(2):383-7. doi: 10.1016/0040-8166(75)90013-0.

Abstract

During fixation of single muscles fibers with glutaraldehyde, the volume of the fiber shrinks 20%, recovers in rinse and osmium tetroxide to near normal volume and shrinks 20% again when staining with uranyl acetate. This suggest that osmotic properties of membranes may not have been completely lost during fixation, post-fixation and en bloc staining. Dehydration in ethanol and propylene oxide produces a further 10% shrinkage in volume. Infiltration and embedding with Epon causes an additional 15% change in volume. This gives a total shrinkage in volume of 45% which is nearly twice that of the apparent shrinkage in the volume of the myosin lattice as determined by electron microscopy.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetates
  • Animals
  • Anura
  • Epoxy Compounds
  • Ethanol
  • Ethers, Cyclic
  • Glutaral
  • Histological Techniques*
  • Microscopy, Electron
  • Muscles / ultrastructure*
  • Myosins
  • Osmium
  • Propane / analogs & derivatives
  • Specimen Handling
  • Staining and Labeling
  • Uranium

Substances

  • Acetates
  • Epoxy Compounds
  • Ethers, Cyclic
  • Osmium
  • Ethanol
  • Uranium
  • Myosins
  • Glutaral
  • Propane
  • propylene oxide