Production of albumin and other serum proteins by clonal cultures of normal human liver

Proc Natl Acad Sci U S A. 1971 Oct;68(10):2396-400. doi: 10.1073/pnas.68.10.2396.

Abstract

Clonal cell strains have been isolated from normal human liver. These cells, while resembling fibroblasts morphologically, function as hepatocytes, as shown by their ability to synthesize and secrete an antigen identical to human serum albumin. Human diploid and aneuploid cell lines from nonliver sources do not exhibit this property. The spectrum of serum proteins synthesized varied from clone to clone and cell line to cell line.

MeSH terms

  • Adult
  • Age Factors
  • Blood Proteins / analysis
  • Blood Proteins / biosynthesis*
  • Carbon Isotopes
  • Cell Division
  • Clone Cells / cytology
  • Clone Cells / metabolism*
  • Complement System Proteins / biosynthesis
  • Epithelial Cells
  • Fetus / cytology
  • Fibrinogen / biosynthesis
  • Fibroblasts
  • Glycoproteins / biosynthesis
  • Haptoglobins / biosynthesis
  • Humans
  • Immunodiffusion
  • Immunoelectrophoresis
  • Infant
  • Lipoproteins / biosynthesis
  • Liver / cytology*
  • Liver / metabolism
  • Macroglobulins / biosynthesis
  • Radioimmunoassay
  • Serum Albumin / biosynthesis
  • Transferrin / biosynthesis

Substances

  • Blood Proteins
  • Carbon Isotopes
  • Glycoproteins
  • Haptoglobins
  • Lipoproteins
  • Macroglobulins
  • Serum Albumin
  • Transferrin
  • Fibrinogen
  • Complement System Proteins