Coding properties of reticulocyte lysine transfer RNA's in hemoglobin synthesis

Science. 1972 Sep 29;177(4055):1197-9. doi: 10.1126/science.177.4055.1197.

Abstract

Two isoacceptor transfer RNA's for lysine were found in rabbit reticulocytes. The codon recognition properties of these isoacceptors were studied in hemoglobin synthesis in a cell-free systemn. The two isoacceptors transferred lysine into different sites in hemoglobin, but showed no preference for one chain over the other. Codon cross recognition was less than 4 percent.

MeSH terms

  • Adenosine / metabolism
  • Animals
  • Carbon Isotopes
  • Cell-Free System
  • Genetic Code*
  • Guanosine / metabolism
  • Hemoglobins / biosynthesis*
  • In Vitro Techniques
  • Lysine / analysis
  • Lysine / metabolism
  • Peptide Biosynthesis
  • Peptides / analysis
  • Protein Binding
  • RNA, Transfer / metabolism*
  • Rabbits
  • Reticulocytes / metabolism*
  • Ribosomes / metabolism
  • Tritium

Substances

  • Carbon Isotopes
  • Hemoglobins
  • Peptides
  • Tritium
  • Guanosine
  • RNA, Transfer
  • Lysine
  • Adenosine