The selection and characterization of an invasive variant of the B16 melanoma

Am J Pathol. 1979 Dec;97(3):587-600.


Several in vitro properties of two variant cell lines of the B16 melanoma (B16-F10 and B16-BL6) with markedly different spontaneous metastatic behavior were examined. The two cell lines were compared with regard to their in vitro growth rate, ability to migrate, ability to adhere to a variety of substrata, detachment rates, production of plasminogen activator, and cell surface proteins as determined by lactoperoxidase-catalyzed iodination. Growth rates in vitro, attachment rates, and qualitative patterns of cell surface proteins were almost identical. B16-F10 cells (the less spontaneously metastatic line) produced greater amounts of plasminogen activator, were more motile in vitro, and detached more readily from plastic than the more invasive B16-BL6 cells. The study of tumor cell variants, selected for different biologic behavior, is a valuable approach to the elucidation of those mechanisms responsible for their malignant activity.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Adhesion
  • Cell Division
  • Cell Line*
  • Cell Movement
  • Melanoma*
  • Membrane Proteins / analysis
  • Mice
  • Mice, Inbred BALB C
  • Neoplasm Metastasis*
  • Neoplasm Proteins / analysis
  • Plasminogen Activators / metabolism


  • Membrane Proteins
  • Neoplasm Proteins
  • Plasminogen Activators