Absence of measurable 2-hydroxyestrone in the rat brain: evidence for rapid turnover

J Clin Endocrinol Metab. 1979 Dec;49(6):940-2. doi: 10.1210/jcem-49-6-940.


The free 2-hydroxyestrone content of female rat brains was measured by two independent methods, including a direct radioimmunoassay and enzymatic conversion to stable O-methylated derivatives followed by a specific radioimmunoassay for the latter. The sensitivities of the two procedures were 10 pg and 5 pg respectively and the recoveries were greater than 85%. Neither assay method was able to detect any measurable endogenous 2-hydroxyestrone in the female rat brain at any stage of the ovulatory cycle. It is suggested that a high turnover rate of 2-hydroxyestrogens in the rat brain precludes the accumulation of detectable quantities of these metabolites in central tissues.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Brain / metabolism
  • Brain Chemistry*
  • Estrone / analogs & derivatives*
  • Female
  • Hydroxyestrones / metabolism*
  • Methylation
  • Radioimmunoassay
  • Rats


  • Hydroxyestrones
  • Estrone