Quantitative analysis of norethindrone in milk using deuterated carrier and gas chromatography-mass spectrometry

J Steroid Biochem. 1979 Sep;11(3):1323-8. doi: 10.1016/0022-4731(79)90203-6.


PIP: A quantitative procedure using deuterated carrier and gas chromatography-mass spectrometry is described and applied to quantitate norethindrone levels in milk from 5 lactating women, aged 20-30 years, who had received 1 silastic implant of 40 mg of norethindrone. The technique is a multiple ion one (gas chromatography-mass spectrometry). The deuterated norethindrone was used as the internal standard and carrier for the detection technique, and, after its addition, the milk samples were extracted with Amberlite XAD-2 columns and purified on Sephadex columns. O-methoxime-trimethylsilyl ether derivatives of the purified samples were analyzed. The assay was precise and reproducible. The mean level of norethindrone at 3 weeks post implant insertion was 208 pg/ml of milk, after which it declined slowly and steadily.

MeSH terms

  • Adult
  • Drug Implants
  • Female
  • Gas Chromatography-Mass Spectrometry
  • Humans
  • Kinetics
  • Milk, Human / analysis*
  • Norethindrone / administration & dosage
  • Norethindrone / analysis*
  • Pregnancy


  • Drug Implants
  • Norethindrone