Improved assay of 3-hydroxy-3-methylglutaryl coenzyme A reductase

J Lipid Res. 1971 Jul;12(4):512-5.

Abstract

Two improvements are described for the assay of HMG CoA reductase. These are a simple synthesis of the substrate precursor HMG-3-(14)C anhydride and a double-label ((14)C and (3)H) method for determining the amount of mevalonate-3-(14)C that is formed from the substrate.

Publication types

  • Comparative Study

MeSH terms

  • Alcohol Oxidoreductases / analysis*
  • Anhydrides
  • Animals
  • Carbon Radioisotopes
  • Chromatography, Paper
  • Chromatography, Thin Layer
  • Evaluation Studies as Topic
  • Glutarates
  • Isotope Labeling
  • Methods
  • Mevalonic Acid
  • Microsomes, Liver / enzymology
  • Tritium
  • Ultracentrifugation

Substances

  • Anhydrides
  • Carbon Radioisotopes
  • Glutarates
  • Tritium
  • Alcohol Oxidoreductases
  • Mevalonic Acid