Abstract
Two improvements are described for the assay of HMG CoA reductase. These are a simple synthesis of the substrate precursor HMG-3-(14)C anhydride and a double-label ((14)C and (3)H) method for determining the amount of mevalonate-3-(14)C that is formed from the substrate.
MeSH terms
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Alcohol Oxidoreductases / analysis*
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Anhydrides
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Animals
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Carbon Radioisotopes
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Chromatography, Paper
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Chromatography, Thin Layer
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Evaluation Studies as Topic
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Glutarates
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Isotope Labeling
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Methods
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Mevalonic Acid
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Microsomes, Liver / enzymology
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Tritium
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Ultracentrifugation
Substances
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Anhydrides
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Carbon Radioisotopes
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Glutarates
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Tritium
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Alcohol Oxidoreductases
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Mevalonic Acid