Activation of mouse complement by different classes of mouse antibody

Immunology. 1979 Dec;38(4):687-95.

Abstract

The capacity of mouse IgM, IgGl, IgG2 and IgA anti-dinitrophenyl (DNP) antibodies to activate mouse or guinea-pig complement was studied, using a sensitive haemolytic assay and two-dimensional immunoelectrophoresis to detect cleavage of mouse C3. Three monoclonal IgM antibodies, and a heterogeneous IgM fraction, lysed trinitrophenylated erythrocytes in the presence of guinea-pig C, but failed to produce lysis in the presence of mouse C. and only activated mouse C3 very inefficiently. A monoclonal IgGl antibody did not produce haemolysis in the presence of guinea-pig or mouse C, but cleaved mouse C3 via the alternative pathway. Two IgA myeloma proteins (M315 and M460) had similar properties. A heterogeneous IgG2 antibody fraction produced haemolysis in the presence of both mouse and guinea-pig C, and was shown to activate both the classical and alternative pathways of mouse C.

MeSH terms

  • Animals
  • Complement C3 / immunology*
  • Complement Fixation Tests
  • Complement System Proteins / immunology*
  • Dinitrobenzenes / immunology
  • Erythrocytes / immunology
  • Guinea Pigs
  • Hemocyanins / immunology
  • Hemolysis
  • Immunoglobulin A
  • Immunoglobulin G
  • Immunoglobulin M
  • Immunoglobulins / classification*
  • Mice
  • Mice, Inbred BALB C
  • Rabbits
  • Sheep
  • Trinitrobenzenes / immunology

Substances

  • Complement C3
  • Dinitrobenzenes
  • Immunoglobulin A
  • Immunoglobulin G
  • Immunoglobulin M
  • Immunoglobulins
  • Trinitrobenzenes
  • Complement System Proteins
  • Hemocyanins