A thrombin-sensitive protein of human platelet membranes

Proc Natl Acad Sci U S A. 1971 Jan;68(1):240-3. doi: 10.1073/pnas.68.1.240.


The action of thrombin on intact human platelets has been studied with the aid of polyacrylamide gel electrophoresis in sodium dodecylsulfate. A single major membrane protein band with a molecular weight of 190,000 disappears after thrombin treatment, while a new membrane protein with a molecular weight of 107,000 appears. This may represent hydrolysis of the thrombin-sensitive protein. When platelets are disrupted or when the thrombin-sensitive protein is solubilized from membranes prior to thrombin treatment, no hydrolysis occurs. The effect of thrombin on the platelet membrane protein is complete within 2 min which suggests that hydrolysis of this membrane protein may trigger the physiological effects of thrombin on platelets.

MeSH terms

  • Blood Platelets / drug effects*
  • Blood Proteins / analysis*
  • Detergents
  • Electrophoresis, Disc
  • Humans
  • Membranes / drug effects
  • Molecular Weight
  • Solubility
  • Sulfuric Acids
  • Thrombin / pharmacology*


  • Blood Proteins
  • Detergents
  • Sulfuric Acids
  • Thrombin