Serum factor requirements of normal and simian virus 40-transformed 3T3 mouse fibroplasts

Proc Natl Acad Sci U S A. 1971 Mar;68(3):645-52. doi: 10.1073/pnas.68.3.645.


Evidence is presented to show the presence in normal rat serum of four different serum factors essential for growth of 3T3 or SV40-transformed 3T3 mouse fibroblasts: a factor that specifically promotes growth of normal 3T3 cells; two factors that specifically promote growth of transformed 3T3 cells; and a factor that sustains viability of both normal and transformed 3T3 cells in serum-free medium, probably without inducing growth of the cells. These factors are separated and partially purified.

MeSH terms

  • Animals
  • Blood Protein Electrophoresis
  • Blood*
  • Cell Transformation, Neoplastic
  • Culture Media*
  • Cytopathogenic Effect, Viral


  • Culture Media