The influence of neighboring base pairs upon base-pair substitution mutation rates

Proc Natl Acad Sci U S A. 1971 Apr;68(4):773-6. doi: 10.1073/pnas.68.4.773.


The 2-aminopurine-induced transition, A.T --> G.C, was studied at particular sites in bacteriophage T4 as a function of the nearby base-pair composition of the DNA. Changing a base pair changed the transition rate at the adjacent base pair up to 23-fold, and at the next base pair by a lesser amount. Destabilization was achieved by replacing an A.T base pair by a G.C base pair.

MeSH terms

  • Adenine / pharmacology
  • Bacteriophages*
  • Base Sequence
  • DNA Replication
  • Mutation* / drug effects
  • Polynucleotides*


  • Polynucleotides
  • Adenine