Ecdysome 20-hydroxylase from the midgut of the tobacco hornworm (Manduca sexta L.)

Experientia. 1976 Apr 15;32(4):438-9. doi: 10.1007/BF01920781.

Abstract

An ecdysone 20-hydroxylase enzyme system that converts alpha-ecdysone to 20-hydroxyecdysone was prepared from the midgut of the tobacco hornworm prepupa. This partially purified enzyme is NADPH dependent and is localized in the mitochondrial fraction of the midgut tissue.

MeSH terms

  • Aging
  • Animals
  • Ecdysone / metabolism*
  • Female
  • Intestines / enzymology*
  • Larva
  • Lepidoptera / enzymology*
  • Male
  • Mitochondria / enzymology
  • Mixed Function Oxygenases / metabolism*
  • Moths / enzymology*
  • NADP / metabolism
  • Pupa

Substances

  • Ecdysone
  • NADP
  • Mixed Function Oxygenases