Application of a simple high-performance liquid chromatographic method for the determination of melphalan in the presence of its hydrolysis products

J Chromatogr. 1979 Sep 14;177(1):91-7. doi: 10.1016/s0021-9673(00)92602-5.


A procedure for the separation and quantitation of melphalan (L-PAM) and its hydrolysis products by high-performance liquid chromatography is described. The hydrolysis of L-PAM at 25 +/- 0.1 degrees and 41 +/- 0.1 degrees was studied between pH 3.0 and 9.0. The pattern of hydrolysis suggested that L-PAM decomposes via two consecutive pseudo first-order reactions. Pseudo first-order rate constants (k1) were determined for the disappearance of L-PAM at various pH values in buffered solutions and in a formulated product. At both temperatures L-PAM solutions were found to be most stable at low pH. Chloride ion was found to reduce the rate of hydrolysis.

MeSH terms

  • Chromatography, High Pressure Liquid / methods
  • Half-Life
  • Hydrolysis
  • Kinetics
  • Melphalan / analysis*


  • Melphalan