Bladder tumors in rats fed cyclohexylamine or high doses of a mixture of cyclamate and saccharin

Science. 1970 Feb 20;167(3921):1131-2. doi: 10.1126/science.167.3921.1131.


Papillary transitional cell tumors were found in the urinary bladders in 8 rats out of 80 that received 2600 milligrams per kilogram of body weight per day of a mixture of sodium cyclamate and sodium saccharin (10:1) for up to 105 weeks. From week 79 on, several of these rats received cyclohexylamine hydrochloride (125 milligrams per kilogram per day, the molecular equivalent of the conversion of about 10 percent of the cyclamate dosage to cyclohexylamine) in addition to the sodium cyclamate and sodium saccharin. In another study in which 50 rats were fed daily 15 milligrams of cyclohexylamine sulfate per kilogram of body weight for 2 years, eight males and nine females survived. One of the eight males had a tumor of the urinary bladder. In neither study were bladder tumors found in the control rats or in rats treated with lower doses of the compounds.

MeSH terms

  • Amines*
  • Animal Feed
  • Animals
  • Body Weight
  • Carcinoma, Papillary / chemically induced*
  • Carcinoma, Transitional Cell / chemically induced*
  • Cyclohexanes*
  • Legislation, Drug
  • Rats
  • Sweetening Agents* / metabolism
  • United States
  • Urinary Bladder Neoplasms / chemically induced*


  • Amines
  • Cyclohexanes
  • Sweetening Agents