Localization of 5S RNA genes on Drosophila chromosomes by RNA-DNA hybridization

Science. 1970 Nov 6;170(3958):639-41. doi: 10.1126/science.170.3958.639.

Abstract

[(3)H]RNA with a high specific activity was prepared from larvae of Drosophila melanogaster grown 4 days in contact with [(3)H]uridine. Purified tritiated 5S RNA was annealed to the DNA of polytene chromosomes, which had been denatured in formamide. The 5S RNA genes are placed within the region 56E-F of the right arm of chromosome 2. This localization was determined from autoradiographs, where the radioactivity from hybrids of [(3)H]RNA and DNA was confined to the 56E-F segment.

MeSH terms

  • Animals
  • Autoradiography
  • Chromosomes / analysis*
  • DNA
  • Drosophila / metabolism*
  • Electrophoresis, Disc
  • Formamides
  • Genes*
  • Hybridization, Genetic
  • Larva
  • Nucleic Acid Denaturation
  • RNA / analysis*
  • RNA / isolation & purification
  • Salivary Glands / cytology
  • Tritium
  • Uridine / metabolism

Substances

  • Formamides
  • Tritium
  • RNA
  • DNA
  • Uridine