The use of an optical brightener in the study of plant structure

Stain Technol. 1975 Sep;50(5):319-29. doi: 10.3109/10520297509117082.


An optical brightener Calcoflour White M2R New has been used to stain cell walls of higher plants. It can be used either as a vital stain for intact plants or for hand sections and plastic-embedded thin sections. Walls are brilliantly fluorescent while most cytoplasmic components are normally unstained. The brightener binds strongly to cellulose, carboxylated polysaccharides, and callose. Staining for 20 sec to 2 min in a 0.01% solution of the brightener is preferred for most purposes.

MeSH terms

  • Cell Wall*
  • Hydrogen-Ion Concentration
  • Plants*
  • Specimen Handling
  • Staining and Labeling*
  • Stilbenes*
  • Triazines*


  • Stilbenes
  • Triazines