Subunit interactions in yeast glyceraldehyde-3-phosphate dehydrogenase

Biochemistry. 1975 Dec 16;14(25):5428-37. doi: 10.1021/bi00696a008.


The spontaneous inactivation of yeast glyceraldehyde-3-phosphate dehydrogenase was found to fit a simple two-state model at pH 8.5 and 25 degrees. The first step is a relatively rapid dissociation of the tetramer to dimers with the equilibrium largely in favor of the tetramer. In the absence of NAD+ the dimer inactivates irreversibly. The apoenzyme is quite stable with a half-life for complete activity loss proportional to the square root of the enzyme concentration. Perturbances of the protein structure (by pH, ionic strength, and specific salts), which have no effect on the tetrameric state of the molecule, result in an alteration of the cooperativity of NAD+ binding, the reactivity of the active-site sulfhydryl group, and the catalytic activity of the enzyme. Covalent modification of two of the four active-site sulfhydryl groups has profound effects on the enzymic activity which are mediated by changes in the subunit interactions. Sedimentation analysis and hybridization studies indicate that the interaction between subunits remains strong after covalent modification. Under normal physiological and equilibrium dialysis conditions the protein is a tetramer. Equilibrium dialysis studies of NAD+ binding to the enzyme at pH 8.5 and 25 degrees reveal a mixed cooperativity pattern. A model consistent with these observations and the observed half-of-the-sites reactivity is that of ligand induced sequential conformational changes which are transferred across strongly interacting subunit domains. Methods for distinguishing negatively cooperative binding patterns from mixtures of denatured enzyme and multiple species are discussed.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Binding Sites
  • Cyanates
  • Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism*
  • Hydrogen-Ion Concentration
  • Kinetics
  • Macromolecular Substances
  • Models, Chemical
  • NAD / metabolism
  • Protein Binding
  • Protein Conformation
  • Protein Denaturation
  • Saccharomyces cerevisiae / enzymology*
  • Sodium Chloride / pharmacology
  • Sulfhydryl Compounds / metabolism


  • Cyanates
  • Macromolecular Substances
  • Sulfhydryl Compounds
  • NAD
  • Sodium Chloride
  • Adenosine Triphosphate
  • Glyceraldehyde-3-Phosphate Dehydrogenases