Separation of two forms of HeLa DNA polymerase alpha with different binding affinity to DNA

Gan. 1978 Apr;69(2):207-12.


Two forms of DNA polymerase alpha were separated from HeLa cells by DEAE-cellulose column chromatography and affinity chromatography using a DNA-cellulose column. They were eluted from a DEAE-cellulose column at 0.22M KCl (P-I) and 0.24M KCl (P-II). Upon cell fractionation between cytosol and nuclei, P-I was recovered from nuclei and P-II from cytosol. P-I was found to have a higher binding affinity to DNA than P-II by DNA-cellulose column chromatography.

MeSH terms

  • Cell Fractionation
  • Cell Nucleus / enzymology
  • Chromatography, Affinity
  • Chromatography, DEAE-Cellulose
  • Cytosol / enzymology
  • DNA / metabolism*
  • DNA-Directed DNA Polymerase / isolation & purification*
  • HeLa Cells
  • Protein Binding
  • Solubility


  • DNA
  • DNA-Directed DNA Polymerase