Primary bioassay of human tumor stem cells

Science. 1977 Jul 29;197(4302):461-3. doi: 10.1126/science.560061.


A simple method has been developed to support human tumor stem cell colony growth in soft agar. The technique appears suitable for culture of a variety of neoplasms of differing histopathology. Tumor stem cell colonies arising from different types of cancer have differing growth characteristics and colony morphology. This bioassay should be suitable for clinical studies of effects of anticancer drugs or irradiation on human tumor stem cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenocarcinoma / pathology
  • Ascites
  • Bone Marrow / pathology
  • Bone Marrow Cells
  • Cells, Cultured*
  • Clone Cells
  • Culture Media
  • Female
  • Humans
  • Lymphoma / pathology
  • Multiple Myeloma / pathology*
  • Neuroblastoma / pathology
  • Ovarian Neoplasms / pathology


  • Culture Media