Stabilization of bovine trypsin by reductive methylation

Biochim Biophys Acta. 1977 Jun 24;492(2):316-21. doi: 10.1016/0005-2795(77)90082-4.


Reductive methylation has little or no detectable effect on the catalytic or physicochemical properties of bovine trypsin but reduces its susceptibility to autolysis. Increased stability after methylation appears to result from the conversion of trypsin-susceptible lysine residues to trypsin-resistant epsilon-N,N-dimethyllysine residues. Reductively methylated trypsin is easily prepared and may be useful in place of trypsin where autolysis is otherwise difficult to control.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cattle
  • Chemical Phenomena
  • Chemistry
  • Drug Stability
  • Enzyme Activation
  • Esterases / metabolism
  • Kinetics
  • Methylation
  • Oxidation-Reduction
  • Trypsin* / metabolism
  • Trypsinogen / metabolism


  • Trypsinogen
  • Esterases
  • Trypsin