Association of DNA polymerase with nucleosomes from mammalian cell chromatin

Eur J Biochem. 1978 Mar;84(1):95-102. doi: 10.1111/j.1432-1033.1978.tb12145.x.


More than half of the DNA polymerase beta in mouse ascites cell chromatin was found to be associated with monomeric nucleosomal particles (produced by micrococcal nuclease treatment of chromatin). Almost all nuclear DNA polymerase activity in lymphocytes was found to be associated with nucleosomes. The nucleosome-associated enzyme was mainly DNA polymerase beta in chromatin from resting and mainly DNA polymerase alpha in chromatin from concanavalin-A-stimulated lymphocytes.

MeSH terms

  • Animals
  • Carcinoma, Ehrlich Tumor / enzymology*
  • Chromatin / drug effects
  • Chromatin / enzymology*
  • Concanavalin A / pharmacology
  • DNA-Directed DNA Polymerase / isolation & purification
  • DNA-Directed DNA Polymerase / metabolism*
  • Deoxyribonucleases
  • Ethylmaleimide / pharmacology
  • Histones*
  • Lymphocytes / enzymology
  • Sodium Chloride / pharmacology


  • Chromatin
  • Histones
  • Concanavalin A
  • Sodium Chloride
  • DNA-Directed DNA Polymerase
  • Deoxyribonucleases
  • Ethylmaleimide