Abstract
Rotenone effectively inhibits the in vitro formation of microtubules from tubulin containing or lacking microtubule-associated proteins. In both cases a concentration of rotenone equal to that of tubulin present completely blocks assembly. The inhibition can be reversed by the addition of dimethylsulfoxide or by removing rotenone with charcoal.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Cattle
-
Cell-Free System
-
Charcoal / pharmacology
-
Colchicine / antagonists & inhibitors
-
Dimethyl Sulfoxide / pharmacology
-
Dose-Response Relationship, Drug
-
Glycoproteins / metabolism*
-
Protein Binding / drug effects
-
Rotenone / antagonists & inhibitors
-
Rotenone / pharmacology*
-
Tubulin / metabolism*
Substances
-
Glycoproteins
-
Tubulin
-
Rotenone
-
Charcoal
-
Colchicine
-
Dimethyl Sulfoxide