Rotenone inhibition of tubulin self-assembly

Biochim Biophys Acta. 1978 Nov 1;543(4):590-4. doi: 10.1016/0304-4165(78)90315-x.

Abstract

Rotenone effectively inhibits the in vitro formation of microtubules from tubulin containing or lacking microtubule-associated proteins. In both cases a concentration of rotenone equal to that of tubulin present completely blocks assembly. The inhibition can be reversed by the addition of dimethylsulfoxide or by removing rotenone with charcoal.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cattle
  • Cell-Free System
  • Charcoal / pharmacology
  • Colchicine / antagonists & inhibitors
  • Dimethyl Sulfoxide / pharmacology
  • Dose-Response Relationship, Drug
  • Glycoproteins / metabolism*
  • Protein Binding / drug effects
  • Rotenone / antagonists & inhibitors
  • Rotenone / pharmacology*
  • Tubulin / metabolism*

Substances

  • Glycoproteins
  • Tubulin
  • Rotenone
  • Charcoal
  • Colchicine
  • Dimethyl Sulfoxide