Establishment of a cell line and its clonal sublines from a patient with hepatoblastoma

Gan. 1976 Feb;67(1):1-10.


By cloning from cultivation in vitro of a human hepatoblastoma 3 epithelial cell lines were established, all of which produced alpha-fetoprotein and one of which did albumin in culture. Observation with electron microscopy demonstrated desmosomes and glycogen granules in the cytoplasm of these cells. The morphology, growth characteristics, alpha-fetoprotein production, heterotransplantability, and karyology of the cloned cells suggested that these clonal lines were derived from the tumor cells and retained their malignant characteristics.

MeSH terms

  • Albumins / analysis
  • Animals
  • Carcinoma, Hepatocellular / analysis
  • Carcinoma, Hepatocellular / pathology*
  • Cell Line
  • Clone Cells* / ultrastructure
  • Cricetinae
  • Cytoplasmic Granules / ultrastructure
  • Humans
  • Infant
  • Karyotyping
  • Liver Neoplasms / analysis
  • Liver Neoplasms / pathology*
  • Male
  • Neoplasm Transplantation
  • Transplantation, Heterologous
  • alpha-Fetoproteins / analysis


  • Albumins
  • alpha-Fetoproteins