New method for obtaining IgA-specific protease

J Immunol Methods. 1977;18(3-4):245-9. doi: 10.1016/0022-1759(77)90178-8.


A simple method for obtaining an active preparation of IgA-specific protease from a bacterial source is presented. In this method Streptococcus sanguis was inoculated onto the surface of a dialysis membrane on nutrient agar. Following growth, the membrane was removed from the agar surface and washed in a small volume of buffer. A solution with protease activity against IgA1 monoclonal proteins was obtained by clarification of the wash and appeared to be similar to enzyme preparations obtained by other methods.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibody Specificity*
  • Electrophoresis, Polyacrylamide Gel
  • Immunoelectrophoresis
  • Immunoglobulin A*
  • Peptide Hydrolases / immunology*
  • Streptococcus sanguis / immunology


  • Immunoglobulin A
  • Peptide Hydrolases