Impaired enteric degradation of pancreatic endopeptidases in antibiotic-treated rats

Scand J Gastroenterol. 1977;12(7):801-9. doi: 10.3109/00365527709181723.


Conventional Sprague-Dawley rats were fed by gastric tube for 5 days with either benzylpenicillin, ampicillin, doxycycline, or clindamycin. In contrast to the pretreatment period fibrinolytic activity and active and immunoreactive trypsin and immunoreactive elastase were present in fecal extracts after 4 days of antibiotic administration. This is consistent with findings in germfree rats and represents an alteration in the intestinal microflora. The germfree characteristics persisted until a suspension of cecal contents from normal rats was administered by enema on the 26th day. In fecal extracts from the clindamycin-treated rats considerable amounts of active and immunoreactive enzymes were, however, still found 10 days after the enema. A possible explanation is that clindamycin or a metabolite remains in the intestinal tract for a long time. The pathophysiological significance of this finding is unknown.

MeSH terms

  • Ampicillin / pharmacology
  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Chromatography, Gel
  • Chymotrypsin* / analysis
  • Chymotrypsin* / immunology
  • Clindamycin / pharmacology
  • Doxycycline / pharmacology
  • Feces / enzymology
  • Intestines / enzymology*
  • Male
  • Pancreatic Elastase* / analysis
  • Pancreatic Elastase* / immunology
  • Penicillin G / pharmacology
  • Rats
  • Trypsin* / analysis
  • Trypsin* / immunology


  • Anti-Bacterial Agents
  • Clindamycin
  • Ampicillin
  • Chymotrypsin
  • Pancreatic Elastase
  • Trypsin
  • Doxycycline
  • Penicillin G