Horizontal polyacrylamide gradient gel electrophoresis for the simultaneous phenotyping of transferrin, post-transferrin, albumin and post-albumin in the blood plasma of cattle

Anim Blood Groups Biochem Genet. 1977;8(3):127-37. doi: 10.1111/j.1365-2052.1977.tb01637.x.

Abstract

A simple method of horizontal polyacrylamide gel electrophoresis was described for the simultaneous phenotyping of transferrin, post-transferrin, albumin and post-albumin in the blood plasma of cattle. A step gradient gel of 8, 4, 12 and 14% acrylamide concentration was used. The method enabled the detection of a new protein polymorphism in the post-transferrin region. Two alleles were observed. The transferrin phenotypes involving D1 and D2 alleles were clearly separated. The resolution of the post-albumin fractions was also better than described by earlier methods.

MeSH terms

  • Alleles
  • Animals
  • Blood Protein Electrophoresis / methods
  • Blood Proteins / genetics
  • Cattle
  • Electrophoresis, Polyacrylamide Gel / methods
  • Phenotype
  • Polymorphism, Genetic
  • Serum Albumin, Bovine / genetics*
  • Transferrin / genetics*

Substances

  • Blood Proteins
  • Transferrin
  • Serum Albumin, Bovine