Observations on template-specific conditions for DNA synthesis by avian myeloblastosis virus DNA polymerase

Nucleic Acids Res. 1976 Jun;3(6):1473-86. doi: 10.1093/nar/3.6.1473.


The effects of Mg++, Mn++, and KCl addition, individually and in combination, on the rate of DNA- and RNA-primed DNA synthesis by avian myeloblastosis virus DNA polymerase (reverse transcriptase) using a variety of natural and synthetic template-primer combinations were examined. Optimal divalent cation concentrations were found to vary by as much as 10-fold depending upon the template-primer used to direct synthesis. Addition of KCl to reaction mixtures containing optimal divalent cation concentrations produced stimulation or inhibition of DNA synthesis which was also template-specific. DNA synthesis on the modified template poly (2'-0-methylcytidylate) was uniquely stimulated by combinations of divalent cations. With Mg++ as divalent cation, deviations from classical Michaelis-Menten kinetics of substrate saturation were observed with all template-primers tested.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Avian Leukosis Virus / enzymology*
  • Avian Myeloblastosis Virus / enzymology*
  • DNA, Viral / biosynthesis*
  • Kinetics
  • Magnesium / pharmacology
  • Manganese / pharmacology
  • Polynucleotides / metabolism
  • Potassium Chloride / pharmacology
  • RNA-Directed DNA Polymerase / metabolism*
  • Templates, Genetic


  • DNA, Viral
  • Polynucleotides
  • Manganese
  • Potassium Chloride
  • RNA-Directed DNA Polymerase
  • Magnesium