The transposon Tn5 carries a bleomycin-resistance determinant

Gene. 1984 Dec;32(1-2):225-33. doi: 10.1016/0378-1119(84)90050-7.


Transposon Tn5 carries a determinant for resistance to bleomycin (Bm). Deletion mapping and cloning experiments have shown that this determinant, gene ble, is located between the determinant for kanamycin (Km) and neomycin resistance (gene neo) and the determinant for streptomycin resistance (gene str). Genes neo, ble, and str belong to an operon controlled by the common promoter. The Mr of the ble product, as determined by polyacrylamide gel electrophoresis, is 12000 to 13000.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics*
  • Bleomycin / pharmacology*
  • Chromosome Mapping
  • Chromosomes, Bacterial
  • DNA Transposable Elements*
  • Drug Resistance, Microbial
  • Escherichia coli / drug effects
  • Escherichia coli / genetics*
  • Genes
  • Genes, Bacterial
  • Operon


  • Bacterial Proteins
  • DNA Transposable Elements
  • Bleomycin