The effect of dexamethasone on parathyroid hormone stimulation of adenylate cyclase in ROS 17/2.8 cells

Endocrinology. 1984 Sep;115(3):951-8. doi: 10.1210/endo-115-3-951.


Treatment of ROS 17/2.8 osteosarcoma-derived cells with dexamethasone potentiates the PTH stimulation of adenylate cyclase in these cells, yielding a detectable response to as little as 10 pM PTH. Isoproterenol stimulation was also enhanced. The dexamethasone effect is first apparent at 12 h and increases with time of treatment. The apparent EC50 for dexamethasone is 3 nM. Hydrocortisone and corticosterone act similarly to dexamethasone, but require 30-fold higher concentrations. Dexamethasone treatment produces no change in high affinity phosphodiesterase activity. Glucocorticoid-potentiating effects are much more pronounced in whole cells than in broken cells and do not influence forskolin stimulation. Particulate fractions of dexamethasone-treated cells have higher adenylate cyclase specific activity, but are stimulated by guanyl-5'-yl imidodiphosphate to the same extent as control cells. These findings suggest that the glucocorticoids potentiate hormone responsiveness through promotion of hormone receptor-adenylate cyclase coupling by a mechanism dependent on cellular integrity.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3',5'-Cyclic-AMP Phosphodiesterases / metabolism
  • Adenylyl Cyclases / metabolism*
  • Animals
  • Cell Line
  • Colforsin
  • Corticosterone / pharmacology
  • Cyclic AMP / metabolism
  • Dexamethasone / pharmacology*
  • Diterpenes / pharmacology
  • Dose-Response Relationship, Drug
  • Guanylyl Imidodiphosphate / pharmacology
  • Hydrocortisone / pharmacology
  • Isoproterenol / pharmacology
  • Mice
  • Osteosarcoma / enzymology*
  • Parathyroid Hormone / pharmacology*
  • Time Factors


  • Diterpenes
  • Parathyroid Hormone
  • Colforsin
  • Guanylyl Imidodiphosphate
  • Dexamethasone
  • Cyclic AMP
  • 3',5'-Cyclic-AMP Phosphodiesterases
  • Adenylyl Cyclases
  • Isoproterenol
  • Corticosterone
  • Hydrocortisone