Characterization and immunocytochemical application of monoclonal antibodies against enkephalins

J Histochem Cytochem. 1984 Sep;32(9):947-57. doi: 10.1177/32.9.6086744.


Monoclonal antibodies were produced following immunization of mice with either [Leu5]enkephalin-bovine serum albumin or [Met5]enkephalin-keyhold limpet hemocyanin conjugates. Two monoclonal antibodies coded NOC1 and NOC2, respectively, were derived. These monoclonal antibodies did not discriminate between Leu- and Met-enkephalin in either radioimmunoassay or immunocytochemistry. NOC1 was characterized in detail. In radioimmunoassay NOC1 displayed about 40% crossreactivity with C-terminal extended Met-enkephalin hexapeptides and 7% with the extended heptapeptide (-Arg-Phe-OH), but did not recognize other endogenous peptides. In immunocytochemistry the NOC1 and NOC2 recognized all well-established "enkephalin immunoreactive sites," but they did not bind to areas known to contain beta-endorphin or high levels of pro-enkephalin. NOC1 was shown to be a suitable tool to demonstrate enkephalin immunoreactive sites by radioimmunocytochemistry utilizing both internally and externally labeled monoclonal antibodies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal*
  • Antigen-Antibody Complex
  • Brain Chemistry
  • Brain Stem / pathology
  • Cross Reactions
  • Endorphins / analysis
  • Enkephalin, Leucine / analysis
  • Enkephalin, Methionine / analysis
  • Enkephalins / analysis*
  • Humans
  • Immunoenzyme Techniques
  • Mice
  • Mice, Inbred BALB C
  • Radioimmunoassay
  • Rats
  • Rats, Inbred Strains
  • Structure-Activity Relationship
  • beta-Endorphin


  • Antibodies, Monoclonal
  • Antigen-Antibody Complex
  • Endorphins
  • Enkephalins
  • Enkephalin, Methionine
  • Enkephalin, Leucine
  • beta-Endorphin