Serum-free medium for generation and propagation of functional human cytotoxic and helper T cell clones

J Immunol Methods. 1984 Aug 3;72(1):219-27. doi: 10.1016/0022-1759(84)90450-2.


A serum-free lymphocyte culture medium is described in which serum is replaced by bovine serum albumin, transferrin, insulin, ethanolamine and a mixture of saturated and unsaturated fatty acids (linoleic acid, oleic acid and palmitic acid). In this serum-free medium proliferative and cytotoxic responses induced in mixed lymphocyte culture were comparable with those obtained in medium containing serum. Antigen-specific cytotoxic and helper T cells were isolated and could be propagated in serum-free medium without loss of function.

MeSH terms

  • Blood Physiological Phenomena
  • Cell Line
  • Cell Separation
  • Cell Transformation, Viral
  • Clone Cells / immunology
  • Culture Media*
  • Cytotoxicity Tests, Immunologic / methods
  • Herpesvirus 4, Human / physiology
  • Humans
  • Lymphocyte Activation*
  • Lymphocyte Culture Test, Mixed / methods
  • T-Lymphocytes, Cytotoxic / immunology*
  • T-Lymphocytes, Helper-Inducer / immunology*


  • Culture Media