Analysis of P transposable element functions in Drosophila

Cell. 1984 Aug;38(1):135-46. doi: 10.1016/0092-8674(84)90534-8.


We have made a P-element derivative called Pc[ry], which carries the selectable marker gene rosy, but which acts like a nondefective, intact P element. It transposes autonomously into the germline chromosomes of an M-strain Drosophila embryo and it mobilizes in trans the defective P elements of the singed-weak allele. Frameshift mutations introduced into any of the four major open reading frames of the P sequence were each sufficient to eliminate the transposase activity, but none affected signals required in cis for transposition of the element. Complementation tests between pairs of mutant elements suggest that a single polypeptide comprises the transposase. We have examined transcripts of P elements both from natural P strains and from lines containing only nondefective Pc[ry] elements, and have identified two RNA species that appear to be specific for autonomous elements.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Animals
  • Base Sequence
  • Crosses, Genetic
  • DNA Restriction Enzymes
  • DNA Transposable Elements*
  • Drosophila / genetics*
  • Female
  • Genes*
  • Genetic Complementation Test
  • Male
  • Mutation
  • Nucleic Acid Hybridization


  • DNA Transposable Elements
  • DNA Restriction Enzymes