Nucleotide sequence and genome organization of foot-and-mouth disease virus

Nucleic Acids Res. 1984 Aug 24;12(16):6587-601. doi: 10.1093/nar/12.16.6587.


A continuous 7802 nucleotide sequence spanning the 94% of foot and mouth disease virus RNA between the 5'-proximal poly(C) tract and the 3'-terminal poly(A) was obtained from cloned cDNA, and the total size of the RNA genome was corrected to 8450 nucleotides. A long open reading frame was identified within this sequence starting about 1300 bases from the 5' end of the RNA genome and extending to a termination codon 92 bases from its polyadenylated 3' end. The protein sequence of 2332 amino acids deduced from this coding sequence was correlated with the 260 K FMDV polyprotein. Its processing sites and twelve mature viral proteins were inferred from protein data, available for some proteins, a predicted cleavage specificity of an FMDV encoded protease for Glu/Gly(Thr, Ser) linkages, and homologies to related proteins from poliovirus. In addition, a short unlinked reading frame of 92 codons has been identified by sequence homology to the polyprotein initiation signal and by in vitro translation studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Aphthovirus / genetics*
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • Computers
  • Cricetinae
  • DNA / analysis
  • DNA Restriction Enzymes
  • DNA, Viral / genetics*
  • Genes, Viral*
  • Kidney
  • Poliovirus / genetics
  • Protein Biosynthesis
  • Viral Proteins / genetics


  • DNA, Viral
  • Viral Proteins
  • DNA
  • DNA Restriction Enzymes

Associated data

  • GENBANK/X00871