Human apolipoprotein A-II: complete nucleic acid sequence of preproapo A-II

FEBS Lett. 1984 Sep 17;175(1):159-64. doi: 10.1016/0014-5793(84)80590-6.


The complete cDNA nucleic acid sequence of preproapolipoprotein (apo) A-II, a major protein constituent of high density lipoproteins, has been determined on clones from a human liver ds-cDNA library. Clones containing ds-cDNA for apoA-II were identified in the human liver ds-cDNA library using synthetic oligonucleotides as probes. Of 3200 clones screened, 4 reacted with the oligonucleotide probes. The DNA sequence coding for amino acids -17 to +17 of apoA-II were determined by Maxam-Gilbert sequence analysis of restriction fragments isolated from one of these clones, pMDB2049. The remainder of the cDNA sequence was established by sequence analysis of a primer extension product synthesized utilizing a restriction fragment near the 5'-end of clone pMDB2049 as primer with total liver mRNA. The apoA-II mRNA encodes for a 100 amino acid protein, preproapoA-II that has an 18 amino acid prepeptide and a 5 amino acid propeptide terminating with a basic dipeptide (Arg-Arg) at the cleavage site to mature apoA-II.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Amino Acid Sequence
  • Apolipoproteins A / genetics*
  • Base Sequence
  • Cloning, Molecular*
  • DNA / metabolism
  • DNA Restriction Enzymes
  • Female
  • Genes*
  • Humans
  • Liver / metabolism
  • Plasmids
  • Protein Precursors / genetics*
  • RNA, Messenger / genetics


  • Apolipoproteins A
  • Protein Precursors
  • RNA, Messenger
  • preproapolipoprotein A-I
  • preproapolipoprotein A-II
  • DNA
  • DNA Restriction Enzymes

Associated data

  • GENBANK/K01686
  • GENBANK/K02216
  • GENBANK/X00569
  • GENBANK/X00927