Cytotoxic effects of WR-2721 on mouse testicular cells

Int J Radiat Oncol Biol Phys. 1984 Sep;10(9):1551-4. doi: 10.1016/0360-3016(84)90501-7.

Abstract

WR-2721 (S-2-(3-aminopropylamino)ethylphosphorothioic acid) has been demonstrated to be cytotoxic to stem spermatogonia in the mouse. Five and 10 injections of 300 mg/kg killed sufficient numbers of stem cells to reduce sperm production 56 days after treatment by 16 and 43%, respectively. Single injections of 300 or 400 mg/kg of WR-2721 given 15 min after irradiation produced negligible toxicity to stem cells as measured by counts of repopulated tubules; 600 mg/kg reduced stem cell survival by 47%. Four daily injections of 300 mg/kg given 4, 3, 2, and 1 days prior to irradiation (with or without a fifth injection 15 min after irradiation) reduced stem cell survival by about 60%. The cytotoxic effects of WR-2721 on testicular stem cells at least partially explains the reduced protection factors observed in the testis with low doses of radiation and during fractionated treatments involving multiple injections of drug.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amifostine / toxicity*
  • Animals
  • Cell Survival / drug effects
  • Cell Survival / radiation effects
  • Dose-Response Relationship, Radiation
  • Male
  • Mice
  • Mice, Inbred C3H
  • Organothiophosphorus Compounds / toxicity*
  • Radiation-Protective Agents / toxicity*
  • Specific Pathogen-Free Organisms
  • Spermatogonia / drug effects*
  • Spermatozoa / drug effects*
  • Stem Cells / drug effects

Substances

  • Organothiophosphorus Compounds
  • Radiation-Protective Agents
  • Amifostine