A human IFN-beta 1 gene deleted of promoter sequences upstream from the TATA box is controlled post-transcriptionally by dsRNA

Nucleic Acids Res. 1984 Sep 25;12(18):6979-93. doi: 10.1093/nar/12.18.6979.

Abstract

Induction of IFN-beta 1 RNA was studied in the mouse cell line SR117-21E transformed by a BPV episome containing the human IFN-beta 1 gene deleted of promoter sequences upstream from position -40. Nuclei isolated from these cells synthesize constitutively IFN-beta 1 RNA from the partially deleted promoter. The IFN-beta 1 RNA synthesized by nuclei of uninduced SR117-21E cells is similar to that made by nuclei of poly(rI):(rC)-induced cells, but does not accumulate and hence no IFN is produced unless the cells have been treated either by ds RNA or by cycloheximide. We conclude that the IFN-beta 1 gene has, in addition to the transcription control due to upstream promoter sequences, an additional post-transcriptional control acting on mRNA accumulation and linked to sequences close to the TATA box and RNA start site. Both controls are relieved by ds RNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cell Line
  • Cell Nucleus / metabolism
  • Chromosome Deletion
  • Cloning, Molecular
  • DNA Restriction Enzymes
  • DNA, Recombinant / analysis
  • Genes*
  • Humans
  • Interferon Type I / genetics*
  • Mice
  • Nucleic Acid Hybridization
  • Operon*
  • Poly I-C / pharmacology
  • RNA Processing, Post-Transcriptional*
  • RNA, Double-Stranded / genetics*
  • RNA, Messenger / genetics
  • Transcription, Genetic

Substances

  • DNA, Recombinant
  • Interferon Type I
  • RNA, Double-Stranded
  • RNA, Messenger
  • DNA Restriction Enzymes
  • Poly I-C

Associated data

  • GENBANK/X00973