Protein products of the bithorax complex in Drosophila

Cell. 1984 Nov;39(1):163-71. doi: 10.1016/0092-8674(84)90202-2.

Abstract

A sequence from the Ubx 5' exon in the bithorax complex of Drosophila melanogaster was expressed as a fusion protein in bacteria. This protein was used to raise rabbit antisera and monoclonal antibodies. These antibodies detect antigens that, on protein blots and by immunofluorescence on whole mounts of imaginal discs, show the predicted segmental distribution of Ubx products. These products are predominantly, if not totally, localized in the cell nucleus. In the embryonic nervous system nuclei are labeled from the second thoracic segment to the eighth abdominal segment. There is no labeling in homozygous Df bxd100 embryos.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Base Sequence
  • Cloning, Molecular
  • DNA Restriction Enzymes
  • Drosophila melanogaster / genetics*
  • Escherichia coli / genetics
  • Fluorescent Antibody Technique
  • Genes*
  • Immune Sera
  • Nucleoproteins / genetics*
  • Thorax
  • Transcription, Genetic

Substances

  • Antibodies, Monoclonal
  • Immune Sera
  • Nucleoproteins
  • DNA Restriction Enzymes