Organization of the Higher-Order Chromatin Loop: Specific DNA Attachment Sites on Nuclear Scaffold

Cell. 1984 Nov;39(1):223-32. doi: 10.1016/0092-8674(84)90208-3.

Abstract

Data are presented for sequence-specific chromatin-loop organization in histone-depleted nuclei from Drosophila melanogaster Kc cells. We find one loop for each of the tandemly repeated histone gene clusters. The attachment site is localized in the A + T rich H1-H3 spacer on a 657 bp fragment. In the cluster of the hsp70 heat-shock genes, in both control and heat-shocked cells, we find two attachment sites in close proximity upstream of regulatory elements. The transcribed sequences are not associated with the nuclear scaffold in control or in heat-shocked cells. A family of attachment sites related by hybridization to those of the hsp70 genes was discovered.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cell Nucleus / ultrastructure*
  • Chromatin / metabolism
  • Chromatin / ultrastructure*
  • DNA / isolation & purification*
  • DNA Restriction Enzymes
  • Drosophila melanogaster
  • Genes*
  • Histones / genetics*
  • Histones / isolation & purification

Substances

  • Chromatin
  • Histones
  • DNA
  • DNA Restriction Enzymes