Expression of the normal human sis/PDGF-2 coding sequence induces cellular transformation

Cell. 1984 Nov;39(1):89-97. doi: 10.1016/0092-8674(84)90194-6.

Abstract

The human sis proto-oncogene contains the coding sequence for one of two polypeptide chains present in preparations of biologically active human platelet-derived growth factor (PDGF). A human clone, c-sis clone 8, which contains all of the v-sis-related sequences present in human DNA, was transcriptionally inactive when transfected into NIH/3T3 cells. When placed under the control of a retrovirus LTR, the clone was transcribed at levels comparable to that observed in cells transformed by SSV DNA. However, c-sis clone 8 DNA did not express detectable sis/PDGF-2 proteins and lacked biologic activity. A putative upstream exon was identified by its ability to detect the 4.2 kb sis-related transcript in certain human cells. When this sequence was inserted in the proper orientation between the LTR and c-sis clone 8, the chimeric molecule acquired high titered transforming activity, comparable to that of SSV DNA. Transformants containing this construct expressed human sis/PDGF-2 translational products. Thus the normal coding sequence for a human growth factor has transforming activity when expressed in an appropriate assay cell.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cell Transformation, Neoplastic*
  • Cells, Cultured
  • Cloning, Molecular*
  • DNA Restriction Enzymes
  • Genes*
  • Glioma
  • Humans
  • Mice
  • Mice, Inbred Strains
  • Oncogenes*
  • Plasmids
  • Platelet-Derived Growth Factor / genetics*
  • Retroviridae / genetics*
  • Sarcoma Virus, Woolly Monkey / genetics*
  • Transfection

Substances

  • Platelet-Derived Growth Factor
  • DNA Restriction Enzymes

Associated data

  • GENBANK/K01918