Characterization and culture of sinusoidal endothelium from normal rat liver: lipoprotein uptake and collagen phenotype

Gastroenterology. 1984 Dec;87(6):1233-47.


Sinusoidal endothelial cells from adult rat liver have been isolated by centrifugal elutriation and been established in primary culture. Their identification has made use of a novel process of these cells, the sequestration of human acetoacetylated low-density lipoprotein. After its administration in vivo, labeled acetoacetylated low-density lipoprotein within the liver was associated solely with sinusoidal endothelial cells and, when labeled with the stable fluorescent compound 3,3'-dioctadecylindocarbocyanine, provided a means of identifying isolated cells by fluorescence microscopy. In endothelial cell cultures, 90% of the cells were fluorescent and exhibited fenestrae. The collagen phenotype of cultures was assessed by an immunofluorescent approach, which revealed cell-associated type IV collagen only; types I, III, and V were undetectable. In other studies, it was found that these cells lacked factor VIII-R antigen and Weibel-Palade bodies, adding to the evidence that they differ substantially from large-vessel and other capillary endothelia.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Carbocyanines
  • Cell Separation
  • Cells, Cultured
  • Collagen / metabolism*
  • Endothelium / cytology
  • Endothelium / metabolism
  • Endothelium / ultrastructure
  • Fluorescent Dyes
  • Iodine Radioisotopes
  • Lipoproteins / metabolism*
  • Lipoproteins, LDL / metabolism
  • Liver / cytology*
  • Liver / metabolism
  • Liver / ultrastructure
  • Male
  • Microbial Collagenase
  • Microscopy, Electron, Scanning
  • Microscopy, Fluorescence
  • Phenotype
  • Pronase
  • Rats
  • Rats, Inbred Strains


  • Carbocyanines
  • Fluorescent Dyes
  • Iodine Radioisotopes
  • Lipoproteins
  • Lipoproteins, LDL
  • 3,3'-dioctadecylindocarbocyanine
  • Collagen
  • Pronase
  • Microbial Collagenase