New shuttle vectors for Bacillus subtilis and Escherichia coli which allow rapid detection of inserted fragments

Gene. Jul-Aug 1984;29(1-2):21-6. doi: 10.1016/0378-1119(84)90161-6.

Abstract

Two new shuttle vectors have been constructed by fusing the Escherichia coli plasmid pUC9 with the Staphylococcus aureus plasmids pU110 and pC194. The resulting hybrids replicate in both E. coli and Bacillus subtilis and contain seven restriction sites within a part of the lacZ gene. Insertion of foreign DNA into those sites can be easily detected in E. coli and hybrid plasmids can subsequently be transformed into B. subtilis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus subtilis / genetics*
  • Base Sequence
  • Cloning, Molecular*
  • DNA Restriction Enzymes
  • DNA Transposable Elements*
  • Escherichia coli / genetics*
  • Genes
  • Genes, Bacterial
  • Genetic Vectors*
  • Plasmids
  • Staphylococcus aureus / genetics

Substances

  • DNA Transposable Elements
  • DNA Restriction Enzymes