A Quantitative Beta-Galactosidase Alpha-Complementation Assay for Fusion Proteins Containing Human Insulin B-chain Peptides

Gene. Jul-Aug 1984;29(1-2):247-50. doi: 10.1016/0378-1119(84)90185-9.

Abstract

The alpha-complementation reaction was adapted as a quantitative assay for fusion proteins consisting of the B chain of human insulin fused to the C terminus of the truncated beta-galactosidase.

MeSH terms

  • Chromatography, High Pressure Liquid
  • Cloning, Molecular*
  • DNA Restriction Enzymes
  • Galactosidases / genetics*
  • Genes*
  • Genes, Bacterial*
  • Genetic Complementation Test
  • Humans
  • Insulin / genetics*
  • Macromolecular Substances
  • Plasmids
  • beta-Galactosidase / genetics*

Substances

  • Insulin
  • Macromolecular Substances
  • DNA Restriction Enzymes
  • Galactosidases
  • beta-Galactosidase